The Podcast Explain is for everyone interested in DNA/RNA sequencing or technology in general. As Iuliia doesn't have a solid background in sequencing, she keeps asking "simple" - even silly at times - questions and Daniel gives his best to make us understand.
A long time coming, but here it is!
Introduction: https://medlineplus.gov/genetics/understanding/howgeneswork/epigenome/
Mechanisms: https://en.wikipedia.org/wiki/Epigenetics
DNA packaging: https://www.nature.com/scitable/topicpage/dna-packaging-nucleosomes-and-chromatin-310/
Chromatin and gene regulation: https://learn.genetics.utah.edu/content/epigenetics/control/
Histone writers, erasers and readers: https://www.nature.com/articles/emm201711
DNA methylation, DNMTs and SAM: https://www.nature.com/articles/npp2012112
Modified bases (5mC, N6-mA): https://en.wikipedia.org/wiki/DNA_methylation
Active DNA demethylation (TET/TDG/BER): https://www.tandfonline.com/doi/full/10.1080/17501911.2025.2586452
Bisulfite sequencing (WGBS): https://www.illumina.com/techniques/sequencing/methylation-sequencing/bisulfite-sequencing.html
WGBS alignment benchmarking: https://www.sciencedirect.com/science/article/pii/S2001037022003853
Illumina 5-base methylome sequencing: https://www.illumina.com/techniques/sequencing/methylation-sequencing/methylome-genome.html
MethylationEPIC v2.0 array: https://www.illumina.com/products/by-type/microarray-kits/infinium-methylation-epic.html
Infinium 450K datasheet: https://www.illumina.com/content/dam/illumina-marketing/documents/products/datasheets/datasheet_humanmethylation450.pdf
PacBio HiFi methylation detection: https://www.pacb.com/revio/
SMRT direct methylation detection: https://www.pacb.com/publications/direct-detection-of-dna-methylation-during-single-molecule-real-time-sequencing/
Fiber-seq and cancer research: https://www.pacb.com/blog/what-a-clearer-view-of-epigenetics-can-mean-for-cancer-research/
PacBio epigenetics overview: https://www.pacb.com/products-and-services/applications/epigenetics/
Sequencing 101: Epigenetics: https://www.pacb.com/blog/sequencing-101-epigenetics/
Oxford Nanopore modified-base calling: https://nanoporetech.com/applications/investigations/epigenetics-and-methylation-analysis
Nanopore sequencing accuracy: https://nanoporetech.com/platform/accuracy
Nanopore chromatin accessibility (EPI2ME): https://epi2me.nanoporetech.com/chromatin-acc-hg002/
Nanopore 5mC/5hmC detection: https://www.nature.com/articles/s42003-025-07681-0
Ultima Genomics (bisulfite, EM-seq, TAPS+): https://www.ultimagenomics.com/focus-areas/methylation-profiling/
Watchmaker TAPS+: https://www.watchmakergenomics.com/taps.html
Element AVITI methylation sequencing: https://www.elementbiosciences.com/applications/dna-sequencing/methylation-sequencing
MGI DNBSEQ bisulfite sequencing: https://link.springer.com/article/10.1186/s13148-023-01543-4
NEBNext EM-seq v2: https://www.neb.com/en-us/products/e8015-nebnext-enzymatic-methyl-seq-v2-kit
NEBNext enzymatic 5hmC-seq: https://www.neb.com/en-us/products/e3350nebnext-enzymatic-methyl-seq-5hmc-kit
5hmC detection overview: https://www.neb.com/en-us/products/epigenetics/methylation-analysis/5hmc-hydroxymethylation
meCUT&RUN: https://www.epicypher.com/product/cutana-mecutrun-kit-for-dna-methylation-sequencing/
Fiber-seq chromatin profiling: https://www.epicypher.com/fiber-seq-long-read-sequencing/
Fiber-seq + PacBio HiFi: https://www.pacb.com/blog/how-pairing-epicyphers-fiber-seq-with-hifi-sequencing-delivers-an-all-in-one-multiomic-view/
MeDIP: https://www.activemotif.com/catalog/736/medip
LINE-1 global methylation assay: https://www.activemotif.com/catalog/1043/global-dna-methylation-assay-line-1
RRBS: https://www.nature.com/articles/nprot.2010.190
DNA methylation biomarkers in liquid biopsies: https://www.nature.com/articles/s41388-025-03624-5
Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).
Podcast Title: Explain Podcast
Creators: Iuliia Novoselova, Daniel Rickert
Website: https://ngs-cn.de/explain-podcast/
7 Apr 2026
454.bio
The open-source DIY Sequencer was here.
Update: 454.bio is now dead, but the archives still have it:
https://web.archive.org/web/20250723191639/https://454.bio/ (https://web.archive.org/web/20250723191639/https://454.bio/)
Their GitHub:
https://github.com/454bio (https://github.com/454bio)
Elements new VITARI:
https://www.elementbiosciences.com/products/preorder-vitari (https://www.elementbiosciences.com/products/preorder-vitari)
20 Mar 2026
Special: other Nanopore Sequencing
We have the same thing at home!
- yes, but in worse, cheaper or both
Qitan:
https://pmc.ncbi.nlm.nih.gov/articles/PMC8985760/ (https://pmc.ncbi.nlm.nih.gov/articles/PMC8985760/)
https://www.qitantech.com/ (https://www.qitantech.com/)
The QPinnacle2:
https://www.vcbeathealth.com/article/1821 (https://www.vcbeathealth.com/article/1821)
CycloneSEQ, the MGI-internal clone:
https://en.cyclone-seq.com/nanopTech (https://en.cyclone-seq.com/nanopTech)
https://www.prnewswire.com/news-releases/mgi-launches-new-nanopore-sequencing-products-with-advanced-cycloneseq-technology-302241816.html (https://www.prnewswire.com/news-releases/mgi-launches-new-nanopore-sequencing-products-with-advanced-cycloneseq-technology-302241816.html)
https://www.biorxiv.org/content/10.1101/2024.08.19.608720v1 (https://www.biorxiv.org/content/10.1101/2024.08.19.608720v1)
https://rrwick.github.io/2024/12/17/cycloneseq.html (https://rrwick.github.io/2024/12/17/cycloneseq.html)
https://gigabytejournal.com/articles/154 (https://gigabytejournal.com/articles/154)
https://gigasciencejournal.com/blog/open-cycloneseq-benchmarking-for-complete-bacterial-genomes/ (https://gigasciencejournal.com/blog/open-cycloneseq-benchmarking-for-complete-bacterial-genomes/)
Polyseq:
https://polyseq.com/ (https://polyseq.com/)
Axbio:
https://www.axbio.cn/products/45.html (https://www.axbio.cn/products/45.html)
Geneus-tech:
http://www.geneus-tech.com/proddetail.aspx?id=1 (http://www.geneus-tech.com/proddetail.aspx?id=1)
Nice not up-to-date table of specs:
https://github.com/zhangtianyuan666/NanoporeReview2024 (https://github.com/zhangtianyuan666/NanoporeReview2024)
ONT to double promethion throughput: https://www.genomeweb.com/sequencing/jpm-oxford-nanopore-shares-plans-double-flow-cell-output-expand-menu-biopharma-market (https://www.genomeweb.com/sequencing/jpm-oxford-nanopore-shares-plans-double-flow-cell-output-expand-menu-biopharma-market)
Reach and audience
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YouTube views
1,200
Score snapshot 15 Sept 2025
Apple Podcasts (US)
5.0 / 5
1 ratings
Podcast Authority Score: 39 / 100
A composite of feed quality, social presence, YouTube performance and engagement. Read the methodology.
Quality
36
Social presence
0
YouTube
74
Engagement
30
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